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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: May 27, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Selective targeting of parallel G-quadruplex structure using L-RNA aptamer.

Danyang Ji¹, Jia-Hao Yuan², Shuo-Bin Chen²

¹Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.

Nucleic Acids Research

|October 23, 2023

Summary

Researchers developed a new method, G4-SELEX-Seq, to create L-RNA aptamers. The aptamer L-Apt12-6 specifically targets parallel G-quadruplexes (G4), offering therapeutic potential and tools for gene regulation.

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Area of Science:

Molecular Biology
Biochemistry
Therapeutics

Background:

G-quadruplexes (G4) are nucleic acid structures with diverse conformations.
Targeting specific G4 conformations is crucial for therapeutic interventions.
Existing G4-binding tools lack high specificity for particular G4 conformations.

Purpose of the Study:

To introduce a novel method, G4-SELEX-Seq, for aptamer selection.
To develop the first L-RNA aptamer with high selectivity for parallel G4 structures.
To demonstrate the utility of the aptamer in regulating biological processes.

Main Methods:

G4-SELEX-Seq for aptamer selection.
In vitro and cellular binding assays to assess aptamer affinity.
Functional assays to evaluate aptamer's effect on DNA replication and gene expression.

Main Results:

Development of L-Apt12-6, the first L-RNA aptamer targeting parallel G4.
High binding affinity of L-Apt12-6 to parallel dG4 c-kit 1 in vitro and in cells.
Demonstrated applications of L-Apt12-6 in controlling DNA replication and gene expression.

Conclusions:

L-Apt12-6 is a valuable tool for targeting parallel G-quadruplex conformations.
The aptamer can regulate G4-mediated biological processes.
G4-SELEX-Seq is a versatile platform for selecting G4-targeting L-RNA aptamers.