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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Transmission Electron Microscopy01:15

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Related Experiment Video

Updated: Jul 12, 2025

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
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Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

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Square beams for optimal tiling in TEM.

Eugene Yd Chua1, Lambertus M Alink1, Mykhailo Kopylov1

  • 1Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027.

Biorxiv : the Preprint Server for Biology
|October 24, 2023
PubMed
Summary
This summary is machine-generated.

A new square electron beam for transmission electron microscopes enables perfect tiling for large field imaging. This advancement improves dose-sensitive cryo-electron microscopy (cryo-EM) imaging with high resolution.

Keywords:
TEMTEM tilingcryo-EMsquare beam

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Area of Science:

  • Electron microscopy
  • Imaging technology
  • Biophysics

Background:

  • High-magnification imaging of large fields of view necessitates tiling.
  • Conventional transmission electron microscopes with round beams produce imperfect tiling and uneven exposures, problematic for dose-sensitive samples.

Conclusions:

  • A square electron beam offers a significant improvement for large field tiling in electron microscopy.
  • This technology enhances cryo-EM imaging quality, especially for dose-sensitive applications.
  • The ease of retrofitting makes this a practical advancement for existing transmission electron microscopes.