Alternative polyadenylation regulates acetyl-CoA carboxylase function in peanut
Zhenying Peng1, Shuang Yu2, Jingjing Meng3
1Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Science, Jinan, 250100, China. pengzhenying2005@126.com.
BMC Genomics
|October 24, 2023
Summary
Alternative polyadenylation (APA) is widespread in peanut seeds, influencing gene expression and seed maturation. Researchers identified four 3' UTRs for the AhACCA1 gene, with UTR2 significantly impacting gene regulation.
Area of Science:
- Plant molecular biology
- Genomics
- Gene regulation
Background:
- Alternative polyadenylation (APA) generates multiple mRNA transcripts with varying 3' untranslated regions (UTRs) from a single gene.
- APA impacts approximately 60-70% of eukaryotic genes and is crucial for cell proliferation, differentiation, and tumorigenesis.
Purpose of the Study:
- To investigate the role and extent of APA in peanut seed development.
- To characterize the different 3' UTRs of the peanut acetyl-CoA carboxylase A1 (AhACCA1) gene and their regulatory functions.
Main Methods:
- Long-read, single-molecule sequencing of peanut seed mRNA.
- RT-PCR analysis to determine transcript expression patterns.
- Transient expression in Nicotiana benthamiana and functional validation in yeast cells.
Main Results:
- Over half of peanut genes exhibit multiple polyadenylation sites (PASs), with increased complexity in older seeds, indicating tissue specificity and a role in maturation.
- Four distinct 3' UTRs (UTR1-4) were identified for the AhACCA1 gene, each with specific tissue expression patterns.
- UTR2 demonstrated a stronger regulatory effect on AhACCA1 expression in yeast compared to other UTRs and a standard terminator.
Conclusions:
- APA is prevalent in peanut seeds and is vital for seed maturation.
- The AhACCA1 gene possesses multiple 3' UTRs with distinct tissue-specific roles.
- UTR2 plays a significant role in regulating gene expression, highlighting the functional importance of APA in plants.
Keywords:
3′-untranslated regionAcetyl-CoA carboxylaseAhACCA1 geneAlternative polyadenylationPeanutPolyadenylation sitesMore Related Videos
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