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Updated: Jul 12, 2025

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

Engineered live bacteria as disease detection and diagnosis tools.

Imen Tanniche¹, Bahareh Behkam^2,3,4

¹Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.

Journal of Biological Engineering

|October 24, 2023

Summary

Engineered bacteria are emerging as powerful, low-cost biosensors for early disease detection. These living sensors offer sensitive, in situ diagnostics and potential for targeted treatments, revolutionizing medical diagnostics.

Keywords:

Biomedical diagnostics Imaging vectors Living machines Synthetic biology Whole-cell biosensors

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Last Updated: Jul 12, 2025

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

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Published on: May 21, 2015

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Area of Science:

Biotechnology
Synthetic Biology
Medical Diagnostics

Background:

Sensitive and minimally invasive diagnostics are crucial for early disease detection and management.
Engineered bacteria offer a novel approach to biosensing, providing robust, noninvasive, and cost-effective solutions.
Current diagnostic technologies face limitations in detecting certain biomarkers and in situ disease states.

Purpose of the Study:

To review the unique advantages of using bacteria as living sensors for medical diagnostics.
To describe various bacteria-based diagnostic approaches and their applications in disease detection.
To explore the potential of bacteria as imaging vectors and in image-guided surgery.

Main Methods:

Summarizing the benefits of bacteria as living sensors.
Describing bacteria-based diagnosis strategies and providing examples.
Discussing the use of bacteria in medical imaging and image-guided surgery.

Main Results:

Engineered bacteria function as sophisticated biosensors programmed with genetic rules and logic gates.
Bacteria-based whole-cell biosensors (BWCBs) can perform detection, recording, and closed-loop treatment regulation.
Applications span early disease detection, monitoring, and image-guided interventions.

Conclusions:

Bacteria-based diagnostics present significant opportunities for sensitive, in situ disease detection and treatment.
Further research is needed to address challenges and facilitate clinical translation of these innovative systems.
Engineered bacteria hold promise for revolutionizing medical diagnostics and therapeutic strategies.