Application of the fuzzy proportional integral differential (PID) temperature control algorithm in a liver function...

Area of Science:

• Biochemistry
• Microfluidics
• Clinical Diagnostics

Background:

• Liver function is commonly assessed through biochemical analysis of serum markers such as alanine aminotransferase (ALT) and aspartate transaminase (AST).
• Conventional methods for detecting these liver enzymes can be time-consuming and require specialized laboratory equipment.
• There is a need for rapid, portable, and user-friendly diagnostic tools for liver function assessment.

Purpose of the Study:

• To develop a novel centrifugal microfluidic-based system for the efficient detection of liver function markers.
• To integrate sample preparation, reagent handling, and biochemical analysis onto a single microfluidic chip.
• To validate the system's performance for clinical biochemical analysis.

Main Methods:

• A centrifugal microfluidic chip was designed to perform automated blood cell separation and serum extraction using centrifugal force.
• Microchannels and microchambers facilitated the precise extraction and mixing of serum with lyophilized reagent beads.
• A Fuzzy PID algorithm controlled the heating film for optimal reaction temperature, and absorbance was measured at 340 nm according to Beer-Lambert law.

Main Results:

• The system successfully separated blood cells from serum and prepared reaction mixtures on-chip.
• The Fuzzy PID algorithm ensured precise temperature control for enzymatic reactions.
• A standard curve was constructed by measuring the rate of change in absorbance, demonstrating quantitative analysis capability.

Conclusions:

• The developed centrifugal microfluidic system provides a portable, rapid, and user-friendly platform for liver function marker detection.
• This technology represents a significant advancement over conventional biochemical analysis methods.
• The system holds potential for future applications in integrated on-chip point-of-care diagnostic devices.