IRR1 contributes to de novo root regeneration from Arabidopsis thaliana leaf explants

Plants are capable of regenerating adventitious roots (ARs), which is important for plant response to stress and survival. Although great advances in understanding AR formation of leaf explants have been made, the regulatory mechanisms of AR formation still need to be investigated. In this study, irr1-1 (impaired root regeneration) was isolated with the inhibition of adventitious rooting from Arabidopsis leaf explants. The β-glucuronidase (GUS) signals of IRR1pro::GUS in detached leaves could be detected at 2-6 days after culture. IRR1 is annotated to encode a Class III peroxidase localized in the cell wall. The total peroxidase (POD) activity of irr1 mutants was significantly lower than that of the wild type. Detached leaves of irr1 mutants showed enhanced reactive oxygen species (ROS) accumulation 4 days after leaves were excised from seedlings. Moreover, thiourea, a ROS scavenger, was able to rescue the adventitious rooting rate in leaf explants of irr1 mutants. Addition of 0.1 μM indole-3-acetic acid (IAA) improved the adventitious rooting from leaf explants of irr1 mutants. Taken together, these results indicated that IRR1 was involved in AR formation of leaf explants, which was associated with ROS homeostasis to some extent.