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Updated: Jul 12, 2025
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
A miniTurbo-based proximity labeling protocol to identify conditional protein interactomes in vivo in Myxococcus
Marco Herfurth1, Franziska Müller1, Lotte Søgaard-Andersen1
1Department for Ecophysiology, Max-Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.
Abstract:
Protein-protein interactions are foundational for many cellular processes. Such interactions are especially challenging to identify if they are transient or depend on environmental conditions. This protocol details steps to identify stable and transient protein interactomes in the bacterium Myxococcus xanthus using biotin ligase miniTurbo-based proximity labeling. We include instructions for optimizing the expression of control proteins, in vivo biotin labeling of bacteria grown on a surface or in suspension culture, enrichment of biotinylated proteins, and sample processing for proteomic analysis. For complete details on the use and execution of this protocol, please refer to Branon et al. (2018).1.
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