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[Effect of tomicide and the cell wall biopolymers of Streptococcus sp. Thom-1606 on mast cell degranulation]
Abstract:
The influence of tomicide and biopolymers obtained from the cell wall of Streptococcus sp. TOM-1606 on the degranulation of mast cells was studied. Among the biopolymers of the streptococcal cell-wall polysaccharide was shown to induce the highest destruction of mast cells (14.84 +/- 6.8%). The alteration of mast cells under the effect of peptidoglycan and teichoic acid was mildly positive (11.85 +/- 5.8% and 12.1 +/- 6.2%). At the same time the destruction induced by the complex of noninfectious allergen and the patient's serum was 33.2 +/- 3.8% respectively. Other preparations induced destruction on the level of spontaneous degranulation. The study of the action of the allergen-antibody complex in combination with tomicide and biopolymers obtained from the cell wall of Streptococcus sp. TOM-1606 revealed a decrease in the rate of mast cell degranulation almost to the background level (24.7 +/- 0.55% for the allergen-antibody complex and 8.4 +/- 4.2% to 11.8 +/- 5.3% for streptococcal biopolymers).
Insights
Streptococcus sp. biopolymers influenced mast cell degranulation. While some biopolymers caused mild mast cell destruction, they significantly reduced degranulation when combined with allergen-antibody complexes.
Area of Science:
- Immunology
- Microbiology
- Biochemistry
Background:
- Mast cells play a crucial role in allergic responses.
- Bacterial cell wall components can modulate immune cell activity.
Purpose of the Study:
- To investigate the effect of tomicide and Streptococcus sp. TOM-1606 biopolymers on mast cell degranulation.
- To assess the combined impact of these biopolymers with allergen-antibody complexes on mast cells.
Main Methods:
- In vitro study of mast cell degranulation.
- Treatment with tomicide, streptococcal biopolymers (polysaccharide, peptidoglycan, teichoic acid), and allergen-antibody complexes.
- Quantification of mast cell destruction.
Main Results:
- Streptococcal polysaccharide induced significant mast cell destruction (14.84%).
- Peptidoglycan and teichoic acid showed milder effects (around 12%).
- Combined treatment with biopolymers and allergen-antibody complexes markedly reduced mast cell degranulation.
Conclusions:
- Tomicide and Streptococcus sp. biopolymers modulate mast cell degranulation.
- These bacterial components can attenuate allergic reactions mediated by mast cells when used with allergen-antibody complexes.