Relationship between levels of rheumatoid factor isotypes and complement component C3 conversion in blood from...

Conversion of complement component C3 in plasma from rheumatoid arthritis patients was measured by two different methods. One of the methods gives an estimation of C3 conversion by ELISA measurement of neodeterminants present on the C3d moiety; the other method measures C3 split products expressing D, but not C, epitopes by rocket immunoelectrophoresis (RIE) with intermediate anti-C3c gel. Results from 20 RA patients obtained by the two methods did not correlate significantly (R = 0.52, 0.02 less than p less than 0.05). The results were compared to the level of rheumatoid factors (RFs) of IgG, IgM, and IgA class in serum. A significant correlation was found between the concentration of C3d measured by RIE and level of IgG RFs, whereas neither IgM nor IgA RFs showed correlation to complement activation. The results of the ELISA estimation of C3 activation showed no correlation with the RF level.