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Corrected Version: Protection of Mice from Controlled Cortical Impact Injury by Food Additive Glyceryl Tribenzoate.

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Corrected Version: Sodium Benzoate, A Metabolite of Cinnamon and A Food Additive, Improves Cognitive Functions in Mice After Controlled Cortical Impact Injury.

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RETRACTED: Rangasamy et al. Protection of Mice from Controlled Cortical Impact Injury by Food Additive Glyceryl Tribenzoate. <i>Int. J. Mol. Sci.</i> 2023, <i>24</i>, 2083.

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Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
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A simple protocol for isolating microglia from adult mouse brain.

Sudipta Chakrabarti1, Sukhamoy Gorai1, Kalipada Pahan1,2

  • 1Department of Neurological Sciences, Rush University Medical Center, Chicago, USA.

Neuroimmune Pharmacology and Therapeutics
|October 27, 2023
PubMed
Summary

We developed a simple method to isolate functional adult microglia from mouse brains. This technique enables research into neurodegenerative diseases using adult microglia, overcoming limitations of neonatal cell cultures.

Keywords:
activationadult mouse brainmicroglianeurodegenerationphagocytosis

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Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Microglia activation is implicated in neurodegenerative and neuroinflammatory disorders.
  • Neonatal microglia are commonly used in cell culture due to ease of isolation.
  • Adult microglia isolation typically requires complex methods like density gradient centrifugation or magnetic beads.

Purpose of the Study:

  • To present a straightforward protocol for isolating highly purified microglia from adult mouse brains.
  • To demonstrate the functionality of adult microglia obtained through the described method.

Main Methods:

  • Dilution of adult mouse brain tissue with sterile PBS or media.
  • Standard centrifugation steps.
  • Plating of isolated cells on poly-D-lysine-coated flasks.

Main Results:

  • Isolated adult microglia expressed inducible nitric oxide synthase (iNOS) in response to alpha-synuclein fibrils (Parkinson's disease reagent) and lipopolysaccharides (LPS).
  • Adult microglia demonstrated phagocytic activity, which was enhanced by LPS treatment.

Conclusions:

  • The described protocol yields functional adult microglia.
  • These isolated adult microglia are suitable for research on neurodegenerative disorders.