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Novel Universal Bond Containing Bioactive Monomer Promotes Odontoblast Differentiation In Vitro
Yaxin Rao1, Youjing Qiu2, Bayarchimeg Altankhishig1
1Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu 061-0293, Hokkaido, Japan.
Journal of Functional Biomaterials
|October 27, 2023
Summary
A new bioactive universal bond containing calcium 4-methacryloxyethyl trimellitic acid (CMET) significantly enhanced odontoblast differentiation and mineralization in vitro. This novel dental material shows promise for dentin regeneration applications.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Regenerative Dentistry
Background:
- Multifunctional dental materials are crucial for advanced restorative and regenerative therapies.
- Odontoblast differentiation and mineralization are key processes in dentin formation and repair.
Purpose of the Study:
- To evaluate the in vitro effects of a novel universal bond containing a bioactive monomer, CMET, on odontoblast-like cells.
- To assess the impact of the bioactive bond on cell proliferation, differentiation, and mineralization.
Main Methods:
- Rat odontoblast-like cells (MDPC-23) were cultured with eluates from three bonding agents: bioactive universal bond with CMET (BA (+)), bioactive universal bond without CMET (BA (-)), and a commercial control (SC).
- Cell counting kit-8 and alkaline phosphatase assays were used to measure proliferation and differentiation.
- Real-time RT-PCR analyzed the expression of odontogenic markers (DSPP, DMP-1).
- Mineralization was assessed by calcific nodule formation.
Main Results:
- The BA (+) group demonstrated significantly higher cell proliferation and alkaline phosphatase activity compared to BA (-) and SC groups.
- mRNA expression of DSPP and DMP-1 was significantly upregulated in the BA (+) group.
- Calcific nodule formation was dose-dependently accelerated in the BA (+) group, unlike the other groups.
Conclusions:
- The novel bioactive universal bond (BA bond) containing CMET exhibits significant potential for promoting dentin regeneration.
- The presence of CMET in the universal bond is critical for enhancing odontoblast activity and mineralization.
