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Updated: Jul 12, 2025
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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes
Published on: July 28, 2016
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Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section
Belinda L Spillings1, Kathryn Green2, Erica Lovas2
1Institute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia.
STAR Protocols
|October 27, 2023
Summary
This study details a transmission electron microscopy (TEM) protocol to visualize glycan-mediated binding between virus-like particles (VLPs) and human immune cells. This method aids in understanding viral attachment and host cell interactions.
Area of Science:
- Virology
- Immunology
- Microscopy
Background:
- Glycan-glycan interactions are crucial for viral attachment to host cells.
- Understanding these interactions can reveal mechanisms of viral entry and infection.
Purpose of the Study:
- To present a detailed protocol for visualizing glycan-mediated binding between virus-like particles (VLPs) and human peripheral blood mononuclear cells (PBMCs).
- To enable the study of viral-host cell interactions at the ultrastructural level using transmission electron microscopy (TEM).
Main Methods:
- Protocol development for virus and VLP production.
- Isolation of human peripheral blood mononuclear cells (PBMCs).
- Sample preparation and thin-section transmission electron microscopy (TEM) for visualizing glycan-mediated binding.
Main Results:
- A comprehensive protocol for visualizing glycan-mediated binding between VLPs and PBMCs using TEM is established.
- The protocol allows for detailed ultrastructural analysis of viral-host cell interactions.
Conclusions:
- The presented TEM protocol provides a valuable tool for studying glycan-mediated viral attachment to host cells.
- This method can advance the understanding of viral pathogenesis and inform the development of antiviral strategies.
