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Updated: Jul 12, 2025
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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
Published on: May 14, 2015
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Prospective isolation of neural stem and progenitor cells from the developing human brain
Daniel Dan Liu1, Joy Q He1, Nobuko Uchida1
1Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
STAR Protocols
|October 28, 2023
Summary
This study details a new protocol for isolating 10 distinct neural stem and progenitor cell types from the developing human brain using fluorescence-activated cell sorting for functional studies.
Area of Science:
- Neuroscience
- Developmental Biology
- Stem Cell Research
Background:
- Studying stem cells in primary human tissues requires isolating specific cell types.
- Neural stem and progenitor cells are crucial for brain development and function.
Purpose of the Study:
- To present a protocol for purifying distinct neural stem and progenitor cell populations from the developing human brain.
- To enable functional studies of these isolated cell types.
Main Methods:
- Protocol for tissue dissociation, fluorescent staining, and cell sorting using fluorescence-activated cell sorting (FACS).
- Purification of 10 transcriptomically and functionally distinct neural stem and progenitor cell types.
- Downstream functional assays including clonogenicity, differentiation, and engraftment potential.
Main Results:
- Successful isolation of 10 distinct neural stem and progenitor cell populations.
- Demonstration of the protocol's utility for downstream functional analysis.
- Characterization of purified populations based on transcriptomic and functional properties.
Conclusions:
- The presented protocol enables prospective isolation of defined neural stem and progenitor cell types from the developing human brain.
- This method is essential for advancing functional studies of human brain development and stem cell biology.
