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Updated: Jul 12, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
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Tracing multiple scattering trajectories for deep optical imaging in scattering media.

Sungsam Kang1,2, Yongwoo Kwon1,2, Hojun Lee1,2

  • 1Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul, Korea.

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|October 29, 2023
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Summary

This study introduces a novel method to image objects deep within scattering media by utilizing multiple light scattering. The technique amplifies ballistic waves, significantly improving imaging depth and correcting distortions.

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Area of Science:

  • Optics
  • Biomedical Imaging
  • Photonics

Background:

  • Multiple light scattering in complex media obstructs imaging by obscuring ballistic waves.
  • Current methods focus on filtering out scattering, limiting imaging depth and resolution.

Purpose of the Study:

  • To develop a method that deterministically utilizes multiple scattering for enhanced microscopic imaging.
  • To overcome the limitations of current techniques for imaging deep within scattering media.

Main Methods:

  • A stack of complex phase plates was identified to mimic the scattering medium's light trajectories.
  • Inverse scattering principles were applied using the identified phase plates.
  • Multiple scattering was rectified, and ballistic waves were amplified.

Main Results:

  • Ballistic waves were amplified by approximately 600 times.
  • Imaging depth was increased by more than three times the scattering mean free path.
  • Image distortions were corrected, enabling clearer imaging.

Conclusions:

  • The proposed method offers a significant advancement in microscopic imaging through scattering media.
  • This work represents a milestone in addressing high-order inverse scattering problems.
  • The technique enables deeper and clearer imaging of embedded objects.