Related Experiment Video
Updated: Jul 12, 2025

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Published on: January 22, 2021
COMPARATIVE ASSESSMENT OF BACTERIAL PERMEABILITY OF A PERSONAL PROTECTIVE RESPIRATORY EQUIPMENT AT DIFFERENT
Ivan M Klishch1, Andriy O Kovalchuk1, Ihor I Medvid1
1I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE.
Objective:
The aim: To establish the level of antibacterial protection of the studied personal protective respiratory equipment set and its main components and compare antibacterial resistance of the personal protective respiratory equipment set in the presence and absence of filtering components.
Patients And Methods:
Materials and methods: The proposed methodology for assessing biological protection parameters is based on testing the permeability of personal respiratory protection equipment for bacteria by the method of serial dilutions. Also additional culturing of separate components of the protective set on a separate media is carried out. The experiment was also repeated in the absence of filtering elements and when they were replaced by gauze masks.
Results:
Results: The use of a fully equipped pneumatic helmet counteracted the penetration of the bacterial aerosol, which was manifested in the absence of growth on the media. The results obtained with the full configuration, as well as the indicators of the spread of bacteria when removing the filter elements and replacing them with gauze masks, showed that the device creates sufficient positive air pressure inside. The latter becomes a restraining factor that does not allow microorganisms to penetrate through the lower circuit.
Conclusion:
Conclusions: Increasing the duration of continuous operation of the conceptual model up to 24 hours, increasing the bacterial load on the filters do not lead to a deterioration in the properties of antibacterial protection. Bacterial aerosol did not penetrate into the inner space of pneumatic helmet.
More Related Videos
10:26P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
Published on: June 15, 2020
07:46Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
Published on: June 5, 2012
Related Concept Videos
Personal Protective Equipment
Assessment of Respiration
Subjective Assessment: Nurses interview the patient to gather information directly during the subjective assessment. It includes questions about the individual's medical history, medications, and symptoms, focusing on past respiratory conditions like...