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A blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption
Abhi Aggarwal1,2,3,4, Smrithi Sunil2, Imane Bendifallah5
1University of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
We developed T-GECO1, a blue-shifted genetically encoded calcium ion (Ca2+) indicator. This high-performance GECI offers improved two-photon brightness for enhanced neuronal activity imaging.
Area of Science:
- Neuroscience
- Biochemistry
- Molecular Biology
Background:
- Genetically encoded calcium ion (Ca2+) indicators (GECIs) are crucial for monitoring intracellular Ca2+ dynamics in biological systems.
- Existing GECIs have limitations in color palette and photophysical properties, restricting applications in neuronal activity imaging.
- Blue-shifted GECIs offer potential for enhanced two-photon brightness, facilitating multiphoton microscopy.
Conclusions:
- T-GECO1 is a high-performance blue-shifted GECI.
- It offers significant advantages over existing GECIs, particularly late-generation GCaMP variants, under two-photon excitation conditions.
- T-GECO1 expands capabilities for advanced *in vitro* and *in vivo* imaging of neuronal activity.
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