Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
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Updated: Jul 12, 2025
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
Published on: September 26, 2010
Jinyuan Song1, Junlei Zhang1, Yongtao Ji1
1Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China.
Abstract:
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1.
