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Updated: Jul 12, 2025
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
Published on: September 26, 2010
Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations
Jinyuan Song1, Junlei Zhang1, Yongtao Ji1
1Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China.
Abstract:
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1.
