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Updated: Jul 12, 2025
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
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Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression.
Cora N Betsinger1, Joshua L Justice1, Matthew D Tyl1
1Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Washington Road, Princeton, New Jersey, USA.
Msystems
|November 2, 2023
Summary
Human enzyme SIRT2 deacetylase activity supports HCMV replication by regulating cell cycle progression through CDK2 K6 acetylation. This reveals a link between viral infection, protein acetylation, and cell cycle control.
Area of Science:
- Molecular biology
- Virology
- Biochemistry
Background:
- Protein acetylation is a key regulator of protein function in host defense and viral replication.
- The human enzyme SIRT2's role in viral infections is not fully understood.
Purpose of the Study:
- To investigate the role of human enzyme SIRT2 in human cytomegalovirus (HCMV) replication.
- To elucidate the mechanisms by which SIRT2 influences viral replication and host cell cycle progression.
Main Methods:
- Quantitative proteomics
- Flow cytometry cell cycle assays
- Microscopy
- Functional virology assays
Main Results:
- SIRT2 deacetylase activity was found to support HCMV replication.
- SIRT2 regulates CDK2 K6 acetylation, impacting the G1-S cell cycle transition.
- The temporality of SIRT2 functions and substrates during infection was investigated.
Conclusions:
- SIRT2 plays a pro-viral role in HCMV replication.
- SIRT2 links viral infection, protein acetylation, and host cell cycle progression.
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