Related Experiment Video
Updated: Jul 12, 2025

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
The bipartite promoter of Orthonairovirus hazaraense large segment
Naoki Saka1, Keisuke Ohta1, Daniel Kolakofsky2
1Department of Microbiology, School of Medicine, Wakayama Medical University , Wakayama, Japan.
Importance:
The realization that segmented negative-strand RNA virus genome ribonucleoproteins are never free as their RNA ends are always bound to the viral polymerase has highlighted the problem of how these genome segments are replicated and express their mRNAs while their RNA ends remain associated with the polymerase throughout the cycles of RNA synthesis. This study of the length and nucleotide composition of the Orthonairovirus hazaraense L segment-specific double-stranded RNA (dsRNA) promoter element (the promoter duplex) provides insight into how its mRNA might be initiated and suggests that this promoter element acts via its separated single strands as well as via dsRNA.
Related Concept Videos
The Eukaryotic Promoter Region
Non-LTR Retrotransposons
LTR Retrotransposons
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
Leaky Scanning
Viruses with RNA Genomes
Viral Recombination

