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Updated: Jul 11, 2025
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
Desmarestia anceps Montagne modulates inflammatory response in experimental periodontitis in male Wistar rats
Giliano Nicolini Verzeletti1, Eduardo José Gaio2, Keilla Gomes Machado3
1Biotechnology Institute, University of Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Caxias do Sul, RS 95070-560, Brazil; School of Dentistry, Department of Periodontology, University of Caxias do Sul, Rua Francisco Getúlio Vargas, 1130 Bloco S, Caxias do Sul, RS 95070-560, Brazil.
Objective:
Desmarestia anceps Montagne (D. anceps) seaweed has been shown to play a range of biologic activities, such as anti-inflammatory and antioxidant properties. The purpose of this study was to investigate the effects of D. anceps on pathogenesis of ligature-induced experimental periodontitis in rats.
Design:
Fifty-four male Wistar rats were randomly divided into six groups. Doses of D. anceps (100 and 200 mg/kg) were administered for 14 days to animals with or without ligature-induced periodontitis, in order to compare to control groups (vehicle solution). Morphometric registration of alveolar bone loss and histological analysis were conducted. Inflammatory cells were counted on hematoxylin-eosin-stained slides. Immunohistochemical expressions for interleukin-1 beta (IL-1β), tumor necrosis factor (TNF-α), receptor activator of nuclear factor kappa-Β ligand (RANKL) and bone morphogenetic protein 2 (BMP-2) were evaluated.
Results:
Alveolar bone loss was higher in groups exposed to ligature-induced periodontitis, but no significant differences were observed comparing different treatment and control groups. Animals that received D. anceps extracts showed significant lower counts of inflammatory cells. D. anceps promoted increased expression of BMP-2 and inhibited IL-1β, TNF-α and RANKL.
Conclusion:
D. anceps modulates inflammatory response in experimental periodontitis in rats. Further pharmacological and immunological analyses are warranted.
