Related Experiment Video
Updated: Jul 11, 2025
09:43
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
8.6K
Phase variation modulates the multi-phenotypes displayed by clinical Campylobacter jejuni strains
Irene Ortega-Sanz1, Carolina Bocigas1, Beatriz Melero1
1Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain.
Food Microbiology
|November 2, 2023
Summary
Campylobacter jejuni exhibits remarkable resilience, employing diverse strategies like oxygen tolerance and biofilm formation to survive food chain stresses. Phase variation is key to modulating these survival mechanisms.
Area of Science:
- Microbiology and Food Safety
- Bacterial Pathogenesis and Survival
Background:
- High incidence of Campylobacter spp. in the food supply chain necessitates understanding their survival mechanisms.
- Campylobacter jejuni poses a significant challenge due to its ability to withstand harsh environmental conditions.
Purpose of the Study:
- To investigate the survival strategies of clinical Campylobacter jejuni isolates under various stress conditions.
- To analyze the role of specific genes and phase variation in bacterial adaptation and persistence.
Main Methods:
- Phenotypic evaluation of five C. jejuni isolates under different temperatures, atmospheres, and oxidant stresses (hydrogen peroxide, cumene hydroperoxide).
- Assessment of motility, biofilm formation on polystyrene and stainless steel, and aerotolerance.
- Whole Genome Sequencing to identify genes involved in survival mechanisms and phase variation.
Main Results:
- Isolates demonstrated high tolerance to oxygen and peroxide stress, with varying motility and biofilm formation capabilities.
- Aerotolerance correlated with reduced peroxide sensitivity and altered motility, facilitating material-dependent biofilm formation.
- Comparative genomics revealed no distinct gene patterns, but phase variation was critical for modulating survival strategies.
Conclusions:
- Campylobacter jejuni utilizes a combination of complex and adaptable strategies to survive and persist in diverse environments.
- Phase variation plays a crucial role in modulating bacterial survival mechanisms during host infection and environmental exposure.
More Related Videos
05:34Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
Published on: February 23, 2024
1.9K
07:58Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
11.1K
Related Concept Videos
01:23Stringent Response in E. coli
17
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
17
01:30Other Stress Responses in Bacteria
20
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
20
01:18Mutations in Microorganisms
18
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
18
02:53Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K
01:30Development of Antibiotic Resistance
17
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
17
01:26Bacterial Translocation and Protein Secretion
25
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
25