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Updated: Jul 11, 2025
11:39
Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
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Pattern recognition in the landscape of seemingly random chimeric transcripts
Aksheetha Sridhar1, Ankita S More2, Amruta R Jadhav2
1Open Health Systems Laboratory, 9601 Medical Centre Drive, Rockville, MD 20850, US.
Computational and Structural Biotechnology Journal
|November 3, 2023
Summary
Chimeric transcripts (CTs) increase molecular diversity in ovarian cancer cells, contributing to tumor survival. Their formation involves complex genomic and spatial arrangements, suggesting non-random evolutionary mechanisms.
Area of Science:
- Genomics
- Molecular Biology
- Cancer Research
Background:
- Chimeric transcripts (CTs) derived from two genes contribute to tumor cell survival.
- Existing research has gaps in understanding CT formation and diversity.
Purpose of the Study:
- To systematically study the spectrum of CTs in ovarian cancer.
- To elucidate the mechanisms and evolutionary aspects of CT generation.
Main Methods:
- Analysis of RNA sequencing datasets from 160 ovarian cancer samples (The Cancer Genome Atlas).
- Structural annotation of CTs, including partner gene localization and splicing.
- Identification of phenotype-specific associations and evolutionary conservation.
Main Results:
- Complexities in CTs arise from gene localization, splicing, and regulatory regions.
- A mesenchymal signature was dynamically modulated during transformation.
- Protein-coding CTs are conserved; non-coding CTs evolved more recently.
- Genomically distanced genes can form CTs via 3D spatial proximity.
Conclusions:
- CT formation is a non-random process enhancing molecular diversity in cancer cells.
- Spatial gene arrangement is crucial for CT generation, even for distant genes.
- CTs interact with regulatory networks, influencing tumor cell survival.
Keywords:
Chimera formationChimeric transcriptsChromatin architectureEvolutionInter- and intra-chromosomalMesenchymal signatureStructural annotationTransformationMore Related Videos
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