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Published on: January 5, 2017
Association between Urinary-Extracellular-Vesicle-Enriched Proteome Dynamics and Oncological Outcomes Following
Chutimon Promthong1, Raphatphorn Navakanitworakul1,2, Kanokwan Obaun2
1Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.
Computational and Structural Biotechnology Journal
|August 7, 2026
Summary
Monitoring urinary extracellular vesicle proteins during chemoradiation for cervical cancer can predict treatment response and survival. This noninvasive liquid biopsy approach reveals immune suppression in nonresponders and identifies key proteins for prognosis.
Area of Science:
- Oncology
- Proteomics
- Biochemistry
Background:
- Concurrent chemoradiation (CCRT) is standard for locally advanced cervical cancer, but outcomes need improvement.
- Noninvasive monitoring of CCRT response is crucial for better patient management.
Purpose of the Study:
- To investigate temporal proteomic changes in urinary extracellular vesicles (EVs) during CCRT for cervical cancer.
- To identify proteins associated with treatment response and patient survival.
Main Methods:
- Collected longitudinal urine samples from 42 locally advanced cervical cancer patients undergoing CCRT.
- Enriched urinary EVs and performed proteomic profiling using liquid chromatography-tandem mass spectrometry.
- Analyzed differential protein abundance and used time-varying Cox regression for survival analysis.
Main Results:
- Identified 2,352 quantifiable proteins, with 1,055 showing significant temporal shifts.
- Observed increased tumor and virus-associated proteins at 3 months post-CCRT.
- Nonresponders showed suppressed immune pathways and increased protein homeostasis/degradation pathways by 3 months.
- Identified 8 proteins, including immune-related and ribosomal proteins, associated with overall survival.
Conclusions:
- EV-enriched urinary proteomics is a viable noninvasive liquid biopsy for monitoring CCRT dynamics.
- Immune suppression in nonresponders and an 8-protein signature offer insights into survival.
- Findings may guide future studies on adjuvant immune-enhancing therapies for cervical cancer.
