Isophorone-based AIEgens fluorescent probe with red emission for targeting lipid droplets and identifying non-alcoholic fatty liver disease

Due to the disorder of lipid metabolism, the excessive accumulation of lipid droplets (LDs) in liver cells can result in the occurrence of non-alcoholic fatty liver disease (NAFLD). Therefore, it is great of significance to design and synthesized LDs-specific fluorescent probes for the early diagnosis of NAFLD. Herein, we developed a series of aggregation-induced emission (AIE) probes ISO-LD1, ISO-LD2 and ISO-LD3 based on isophorone group for LDs-specific imaging in living cells. The photophysical properties demonstrated that all the probes with red emission (λ_em > 600 nm) exhibited a strong fluorescence in high polarity solvents. In particular, probe ISO-LD3 has a highest fluorescence quantum yield (except for 1,4-dioxane) and a larger Stokes shift. Confocal laser scanning microscopy experiments indicated that probe ISO-LD3 could specifically stain LDs via a "washing-free" procedure within 10 s, and monitor the dynamic behaviors of LDs exhibiting a high signal/noise ratio. Importantly, given the satisfactory performance of probe ISO-LD3, it has been successfully used for the detection of the normal liver tissues and fatty liver tissues, respectively. This work illustrated that ISO-LD3 is a promising tool for the detection of LDs and LDs-related diseases.