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Updated: Jul 11, 2025
Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
Published on: September 25, 2019
CT-based evaluation of tissue expansion in cryoablation of ex vivo kidney
Frank Hübner1, Moritz Klaus1, Norbert Siedow2
1Institute of Diagnostic and Interventional Radiology, University Hospital, Goethe University Frankfurt, Frankfurt, Germany.
Objectives:
To evaluate tissue expansion during cryoablation, the displacement of markers in ex vivo kidney tissue was determined using computed tomographic (CT) imaging.
Methods:
CT-guided cryoablation was performed in nine porcine kidneys over a 10 min period. Markers and fiber optic temperature probes were positioned perpendicular to the cryoprobe shaft in an axial orientation. The temperature measurement was performed simultaneously with the acquisitions of the CT images in 5 s intervals. The distance change of the markers to the cryoprobe was determined in each CT image and equated to the measured temperature at the marker.
Results:
The greatest increase in the distance between the markers and the cryoprobe was observed in the initial phase of cryoablation. The maximum displacement of the markers was determined to be 0.31±0.2 mm and 2.8±0.02 %, respectively.
Conclusions:
The mean expansion of ex vivo kidney tissue during cryoablation with a single cryoprobe is 0.31±0.2 mm. The results can be used for identification of basic parameters for optimization of therapy planning.
