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Updated: Jun 30, 2026
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
Published on: June 21, 2013
Engineering semi-permeable giant liposomes
Sreelakshmi Radhakrishnan1, Karthika S Nair1,2, Samir Nandi1
1Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695019, Kerala, India. harshabajaj@niist.res.in.
Abstract:
Giant unilamellar vesicles (GUVs) with a semi-permeable nature are prerequisites for constructing synthetic cells. Here we engineer semi-permeable GUVs by the inclusion of DOTAP lipid in vesicles. Diffusion of molecules of different charge and size across GUVs are reported. Control over size-selective permeability is demonstrated by modulating the DOTAP lipid composition in different lipid systems without reconstituting membrane proteins. Such semi-permeable GUVs have immense applications for constructing synthetic cells.
