Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activation of the Yersinia type III secretion system induces large-scale spatial reorganization of chromosomal and virulence plasmid DNA.

Cell reports·2025
Same author

A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus.

PLoS genetics·2025
Same author

Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system.

Nature communications·2025
Same author

Exploitation of Biodiversity in Bioeconomy: Examples, Opportunities, and Challenges.

Advances in biochemical engineering/biotechnology·2025
Same author

<i>In situ</i> analysis of type III secretion chaperone proteins indicates a cytosolic handover of virulence effectors.

FEMS microbes·2025
Same author

Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis.

Communications biology·2025
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jul 11, 2025

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

2.7K

Defining Assembly Pathways by Fluorescence Microscopy.

Andreas Diepold1

  • 1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. andreas.diepold@mpi-marburg.mpg.de.

Methods in Molecular Biology (Clifton, N.J.)
|November 6, 2023
PubMed
Summary
This summary is machine-generated.

Researchers used fluorescence microscopy to study how large bacterial secretion systems are built. This method helps understand the assembly pathways and interactions of these complex protein structures in bacteria.

Keywords:
BiogenesisEpistasis experimentsFluorescence microscopyFluorescently labeled proteinsMacromolecular complexesSecretion systemsSubcellular localization

More Related Videos

Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
08:04

Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins

Published on: January 26, 2019

6.9K
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

14.5K

Related Experiment Videos

Last Updated: Jul 11, 2025

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

2.7K
Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
08:04

Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins

Published on: January 26, 2019

6.9K
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

14.5K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biophysics

Background:

  • Bacterial secretion systems are large, complex protein machines essential for bacterial survival and pathogenesis.
  • Understanding their assembly is key to deciphering bacterial cell biology and developing novel antimicrobials.
  • Assembly pathways involve intricate interactions and subcomplex formation.

Purpose of the Study:

  • To elucidate the assembly pathway of bacterial secretion systems.
  • To demonstrate the utility of fluorescence microscopy in studying macromolecular complex biogenesis.
  • To provide insights into the dynamic assembly process of these large protein structures.

Main Methods:

  • Utilizing fluorescence microscopy with fluorescently labeled components.
  • Tracking the spatial and temporal organization of secretion system subunits during assembly.
  • Analyzing the formation of intermediate subcomplexes and their integration into the final structure.

Main Results:

  • Detailed visualization of the step-by-step assembly of bacterial secretion systems.
  • Identification of key intermediate structures and their assembly order.
  • Demonstration of fluorescence microscopy as a powerful tool for dissecting complex biological assembly processes.

Conclusions:

  • Fluorescence microscopy is an effective method for deciphering bacterial secretion system assembly pathways.
  • The study provides crucial insights into the biogenesis and functional organization of these essential bacterial machines.
  • This approach can be applied to study the assembly of other large macromolecular complexes in prokaryotes.