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In-vitro Mutagenesis

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Related Experiment Video

Updated: Jul 11, 2025

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Obtaining or Generating Gene Mutations in Mice.

Virginia E Papaioannou¹, Richard R Behringer²

¹Department of Genetics and Development, Columbia University Medical Center, New York, New York 10032, USA vep1@columbia.edu.

Cold Spring Harbor Protocols

|November 6, 2023

Summary

This summary is machine-generated.

Generating mouse mutants is crucial for gene function studies. This review covers spontaneous, induced, and targeted genome manipulation methods like CRISPR-Cas and homologous recombination for creating specific mutations.

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Last Updated: Jul 11, 2025

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Area of Science:

Genetics
Molecular Biology
Genomics

Background:

Gene function studies rely on the availability of mutant organisms.
Mouse models are essential for understanding mammalian genetics and disease.

Purpose of the Study:

To provide a comprehensive overview of methods for generating mouse mutants.
To compare different genome manipulation techniques for creating specific mutations.
To guide researchers in selecting appropriate methods for their gene function studies.

Main Methods:

Screening for spontaneous mutations.
Chemical or X-ray induced mutagenesis.
Targeted genome manipulation including insertional mutagenesis, homologous recombination in embryonic stem (ES) cells, and CRISPR-Cas gene editing.

Main Results:

Discussion of the advantages and disadvantages of homologous recombination and CRISPR-Cas gene editing.
Overview of resources for obtaining existing mouse mutations.
Guidance for researchers planning gene targeting experiments.

Conclusions:

Multiple strategies exist for generating mouse mutants, ranging from random to targeted approaches.
CRISPR-Cas and homologous recombination offer precise gene editing capabilities.
Researchers can choose the most suitable method based on their specific research needs and available resources.