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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

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Published on: March 18, 2013

Tissue- and/or Temporal-Specific Mutations in Mice Using Conditional Alleles.

Virginia E Papaioannou¹, Richard R Behringer²

¹Department of Genetics and Development, Columbia University Medical Center, New York, New York 10032, USA vep1@columbia.edu.

Cold Spring Harbor Protocols

|November 6, 2023

Summary

This summary is machine-generated.

Conditional alleles offer versatile gene function deletion for developmental and tissue-specific studies. This guide details testing and using these alleles for accurate phenotypic analysis.

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Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

Null alleles are common but limit research on essential genes.
Conditional alleles allow temporal and tissue-specific gene inactivation, overcoming limitations of null mutations.

Purpose of the Study:

To provide a comprehensive overview of testing and utilizing multipurpose conditional alleles.
To guide researchers in ensuring the proper function of engineered conditional alleles and recombinase strains.
To maximize the value of conditional alleles in phenotypic analysis.

Main Methods:

Discusses methods for testing engineered conditional alleles.
Covers testing of recombinase mouse strains, including inducible transgenic and knock-in lines.
Outlines strategies for using conditional alleles in phenotypic analysis.

Main Results:

Conditional alleles enable bypassing early lethal effects by delaying gene inactivation.
Facilitates elimination of indirect effects by enabling tissue- or organ-specific gene deletion.
Ensures desired experimental outcomes through rigorous testing protocols.

Conclusions:

Conditional alleles are valuable tools for precise gene function studies.
Proper testing of alleles and recombinase systems is crucial for reliable results.
Strategic use of conditional alleles enhances the depth of phenotypic analysis.