Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human BMP4 mRNA Encapsulated in Lipid Nanoparticle for Bone and Articular Cartilage Repair in Aged Mice.

Journal of functional biomaterials·2026
Same author

Computational modeling of left ventricular flow using PC-CMR-derived four-dimensional wall motion.

International journal of engineering science·2026
Same author

Investigational New Drug-enabling studies in a human vessel-chip: Are we there yet?

Bioengineering & translational medicine·2026
Same author

Telomerase is induced during wound healing across species.

Skin health and disease·2026
Same author

LATS1/2-CD38 Metabolic Rewiring Links Senescence to Intraplaque Thrombosis.

Circulation research·2026
Same author

A Long-lived Avatar for Modeling Age-Related Vascular Disease.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jul 11, 2025

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
16:27

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Published on: September 14, 2011

12.4K

Disrupted Stiffness Ratio Alters Nuclear Mechanosensing.

Brandon K Walther1,2, Adam P Sears2,3, Anahita Mojiri1

  • 1Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, Houston, TX 77030, USA.

Matter
|November 8, 2023
PubMed
Summary

Aging endothelial cells show altered mechanical properties impacting blood flow sensing. A reduced stiffness ratio between cellular compartments attenuates shear stress transfer, affecting vascular homeostasis and cardiovascular health.

Keywords:
BiomechanicsBiophysicsEndothelial CellsMechanobiologyShear StressSoft Matter

More Related Videos

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
16:46

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

Published on: June 3, 2014

11.8K
Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

3.0K

Related Experiment Videos

Last Updated: Jul 11, 2025

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
16:27

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Published on: September 14, 2011

12.4K
A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
16:46

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

Published on: June 3, 2014

11.8K
Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

3.0K

Area of Science:

  • Cell biology
  • Biophysics
  • Cardiovascular research

Background:

  • Endothelial cells are crucial for vascular homeostasis, sensing blood flow dynamics.
  • Mechanotransduction, the process by which cells sense mechanical stimuli, is vital for cardiovascular health.
  • Nuclear and cytoplasmic properties influence cellular mechanosensory responses.

Purpose of the Study:

  • To investigate how mechanical properties of endothelial cell compartments affect mechanotransduction.
  • To determine the role of nuclear and cytoplasmic stiffness in shear stress transfer.
  • To explore the impact of cellular aging on these mechanosensory mechanisms.

Main Methods:

  • Utilized atomic force microscopy to measure mechanical properties.
  • Employed mathematical modeling and computational studies to analyze shear stress transfer.
  • Compared replicatively aged cells with a genetic model of accelerated aging.

Main Results:

  • The stiffness ratio between nuclear and cytoplasmic compartments, not individual stiffness, critically controls shear stress transfer.
  • Replicatively aged endothelial cells exhibited a reduced stiffness ratio, leading to attenuated shear stress transfer.
  • A genetic model of accelerated aging did not show altered stiffness ratios.

Conclusions:

  • Relative mechanical changes in subcellular compartments can uniquely impair the shear stress response.
  • Dysregulation of mechanotransduction due to altered stiffness ratios may contribute to age-related cardiovascular dysfunction.
  • Findings provide a theoretical framework for understanding mechanosensory consequences of altered cellular mechanics.