Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbiota of the Respiratory Tract01:29

Microbiota of the Respiratory Tract

The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more like...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Health inequalities in Escherichia coli bacteraemia and associated antibiotic resistance: A national surveillance study, England, 2018/19 to 2023/24.

The Journal of infection·2026
Same author

HSV ICP27 hijacks host splicing factor SRSF3 to regulate pre-mRNA splicing and export for viral gene expression and immune evasion.

PLoS pathogens·2026
Same author

Meticillin-resistant Staphylococcus aureus bacteraemia rise in England - start of a troubling trend?

The Journal of hospital infection·2026
Same author

<i>Burkholderia stabilis</i> infections associated with contamination of non-sterile alcohol-free skin cleansing wipes, United Kingdom, 2018 to 2026.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2026
Same author

Antibody escape drives emergence of diverse spike haplotypes resembling variants of concern in persistent SARS-CoV-2 infections.

Cell reports. Medicine·2026
Same author

Enhancing whole genome DNA amplification of Plasmodium falciparum for advanced molecular surveillance in malaria control.

Malaria journal·2025
Same journal

How the Exposome Shapes Our Respiratory Health.

American journal of respiratory and critical care medicine·2026
Same journal

Diagnostic criteria for invasive pulmonary aspergillosis in COPD patients.

American journal of respiratory and critical care medicine·2026
Same journal

Opportunities for Human Lung Tissue-Based Studies in Fibrotic ILD Research.

American journal of respiratory and critical care medicine·2026
Same journal

Astegolimab in COPD: success or failure?

American journal of respiratory and critical care medicine·2026
Same journal

How artificial intelligence could improve the diagnosis and management of COPD: a perspective from GOLD.

American journal of respiratory and critical care medicine·2026
Same journal

Awake ECMO for ARDS: No Vent, No VILI, No Problem?

American journal of respiratory and critical care medicine·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2026

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

37.3K

Routine Metagenomics Service for ICU Patients with Respiratory Infection.

Themoula Charalampous1, Adela Alcolea-Medina1,2, Luke B Snell1,3

  • 1Centre for Clinical Infection and Diagnostics Research, Department of Infectious Diseases, School of Immunology and Microbial Sciences and.

American Journal of Respiratory and Critical Care Medicine
|November 8, 2023
PubMed
Summary
This summary is machine-generated.

Respiratory metagenomics (RMg) shows promise in critical care, offering rapid results that guide antimicrobial prescribing and infection control. This pilot study highlights RMg

More Related Videos

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.0K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.8K

Related Experiment Videos

Last Updated: Jun 30, 2026

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

37.3K
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.0K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.8K

Area of Science:

  • Clinical Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Respiratory metagenomics (RMg) requires evaluation in a service setting to assess its clinical utility.
  • Implementation into routine practice necessitates understanding feasibility, performance, and clinical impact.

Purpose of the Study:

  • To evaluate the feasibility, performance, and clinical impact of a pilot respiratory metagenomics (RMg) service.
  • To assess RMg's influence on antimicrobial prescribing and infection control in lower respiratory tract infections (LRTI).

Main Methods:

  • Respiratory metagenomics (RMg) was performed on 128 samples from 87 patients with suspected LRTI.
  • The study was conducted in general and specialist respiratory intensive care units (ICUs).
  • Feasibility, turnaround time, sensitivity, specificity, and impact on treatment were recorded.

Main Results:

  • RMg provided same-day results for 86% of samples with a median turnaround time of 6.7 hours.
  • RMg demonstrated 93% sensitivity and 81% specificity for clinically relevant pathogens.
  • Forty-eight percent of RMg results led to antimicrobial prescribing changes, including deescalation and escalation based on pathogen identification and resistance genes. Unexpected organisms and virulence factors were identified, impacting treatment and public health.

Conclusions:

  • Pilot RMg testing in critical care settings shows potential benefits for antimicrobial treatment, infection control, and public health.
  • Further multicenter studies are needed to support the translation of RMg into routine clinical services.