Carbon dots with selective fluorescence response to hydroxyl radical for sensitive detection of bleomycin

In this work, antioxidant carbon dots (ACDs) with high quantum yield (63.98%) were developed through a one-step strategy using citric acid, ethylenediamine, and L-arginine as sources. The ACDs demonstrated excellent in vitro antioxidant activity. Additionally, ACDs displayed a unique fluorescence response to hydroxyl radical (OH). Leveraging this distinctive feature, a selective and sensitive fluorescence-based sensor was developed for detecting bleomycin (BLM) in the presence of ferrous ions (Fe²⁺). The studies on the oxidizing properties and dynamic quenching mechanism of ACDs by OH suggested that OH reduced the contents of the amino and hydroxyl groups of ACDs, leading to a decreased electron cloud density of ACDs for dynamic quenching of ACDs. Under optimally refined conditions, the detection limit of this strategy for BLM was 0.58 μg/mL, with a linear range spanning from 1 to 24 μg/mL when employing ACDs as the probe. This fluorescence sensing strategy provides a promising method for the detection of BLM in clinical samples.