Developing an analytical method for quantification of trientine based on modified silver nanoparticles

Area of Science:

• Analytical Chemistry
• Nanotechnology
• Biochemistry

Background:

• Trientine (N,N´-bis(2-aminoethyl)-1,2-ethanediamine, TETA) is a copper chelator crucial for Wilson's disease treatment.
• TETA lacks UV-absorbing groups, posing challenges for direct quantification.
• Silver nanoparticles (AgNPs) offer unique optical properties for developing sensitive analytical methods.

Purpose of the Study:

• To develop and optimize a novel, cost-effective analytical method for quantifying TETA.
• To utilize modified silver nanoparticles (AgNPs) for TETA detection.
• To validate the method's performance in biological matrices like plasma.

Main Methods:

• Modification of AgNPs with sodium lauryl sulfate.
• Optimization of reaction conditions (pH, time, salt, AgNPs volume) using response surface methodology.
• Spectrophotometric measurement of TETA-induced changes in AgNPs' absorbance at 397 nm.

Main Results:

• A linear calibration curve was established for TETA quantification (10-40 ng/mL, R²=0.996).
• The method demonstrated high sensitivity with a limit of detection (LOD) of 3 ng/mL and a limit of quantification (LOQ) of 10 ng/mL.
• The method showed selectivity in plasma, with interference from common cations mitigated by dilution due to high sensitivity.

Conclusions:

• The developed AgNP-based method provides a sensitive, selective, and low-cost approach for TETA quantification.
• This technique is suitable for TETA measurement in plasma without requiring expensive equipment.
• The method offers a practical analytical solution for managing Wilson's disease.