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SPOP regulates the expression profiles and alternative splicing events in human hepatocytes

Jing Dai1, Xiang Dong1,2, Yuxin Chen1

  • 1School of Life Science, Bengbu Medical College, No. 2600 Donghai Road, Bengbu, China.

Open Life Sciences
|November 9, 2023
PubMed

Insights

Speckle-associated protein (SPOP) overexpression appears to promote hepatocellular carcinoma (HCC) by affecting cell proliferation and gene expression. Further research is needed to fully understand SPOP

Area of Science:

  • Molecular Biology
  • Oncology
  • Genomics

Background:

  • The role of Speckle-type POZ protein (SPOP) in hepatocellular carcinoma (HCC) is largely uncharacterized.
  • SPOP's dual potential in cancer necessitates investigation within the context of HCC progression.

Purpose of the Study:

  • To investigate the effects of SPOP in hepatocellular carcinoma (HCC).
  • To elucidate the molecular mechanisms underlying SPOP's role in HCC.
  • To assess SPOP-regulated alternative splicing events in cellular processes.

Main Methods:

  • RNA sequencing was employed to identify differentially expressed genes (DEGs) upon SPOP overexpression.
  • Gene Ontology and KEGG pathway analyses were performed to predict DEG functions.
  • The Cancer Genome Atlas and Gene Expression Omnibus datasets were utilized to correlate SPOP with HCC progression.

Main Results:

  • SPOP overexpression was found to enhance cell proliferation in human normal hepatocytes.
  • Significant alterations in gene expression profiles were observed following SPOP overexpression.
  • SPOP-regulated alternative splicing events were implicated in various cellular and metabolic pathways.

Conclusions:

  • SPOP may exhibit tumor-promoting effects in hepatocellular carcinoma.
  • Further comprehensive investigations are required to fully elucidate the molecular mechanisms of SPOP in HCC.
Keywords:
alternative splicing eventsgene expression profileshepatocellular carcinomahuman hepatocytesspeckle type BTB/POZ protein

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