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Updated: Jul 11, 2025

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
Published on: May 5, 2017
Ice Shell Purification of Ice-Active Compounds
Jessica Morris1, Michelle Liddy2, Craig J Marshall3
1Department of Biochemistry, School of Biomedical Science, Dunedin, New Zealand.
Abstract:
Ice fractionation allows for the selective purification of ice-active compounds from aqueous solutions. A variety of approaches have been developed to allow the interaction of ice with solutions of interest. We describe here a version where a thin layer of ice is formed on the inside of a suitable flask, which is then exposed to solutions containing ice-binding molecules under conditions where the ice shell is allowed to grow slowly. Under these conditions, ice-binding compounds are incorporated into the newly formed ice, and other solutes are accumulated in the remaining liquid fraction.
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