Assessment of coating-efficiency in ELISA plates by direct protein determination

A commercially available protein determination method (Pierce BCA protein determination kit) was used to assess the efficiency of coating of microtiter plates. Since the method is insensitive to detergents it permits the determination of adhered protein after washing the microtiter plate with a detergent-containing solution. Thus only tightly bound protein is determined. Our results suggest that coating of microtiter plates with antibody is optimal at a certain concentration, above which coating is less efficient.