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GlycoTCFM: Glycoproteomics Based on Two Complementary Fragmentation Methods Reveals Distinctive O-Glycosylation in
Mengqi Luo1, Tao Su1, Qingyuan Cheng2
1Department of Nephrology and Institutes for Systems Genetics, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.
Journal of Proteome Research
|November 9, 2023
Summary
This study maps human sperm and seminal plasma O-glycoproteomes, identifying novel O-glycoproteins and glycosites. These findings enhance understanding of O-glycosylation
Area of Science:
- Proteomics
- Glycomics
- Reproductive Biology
Background:
- Protein O-glycosylation is crucial for sperm function and fertilization.
- Human semen is a complex biological fluid with vital roles in reproduction.
Purpose of the Study:
- To create a comprehensive O-glycoproteome map of human sperm and seminal plasma.
- To identify novel O-glycoproteins and their functions in male reproductive processes.
Main Methods:
- Utilized Glycoproteomics based on Two Complementary Fragmentation Methods (GlycoTCFM).
- Developed a database of human sperm and seminal plasma O-glycoproteins.
Main Results:
- Generated the most comprehensive O-glycoproteome map of human sperm and seminal plasma to date.
- Identified 371 intact O-glycopeptides and 202 O-glycosites from 68 O-glycoproteins.
- Reported 105 novel O-glycosites, primarily modified by core 1 O-glycans, and highlighted semenogelin-1, -2, and equatorin.
Conclusions:
- This study significantly expands knowledge of O-glycosylation in human reproduction.
- Provides a foundation for investigating O-glycoprotein roles in male infertility.
