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Updated: Jul 11, 2025
07:58
qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
Published on: March 6, 2019
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Underrepresentation of activating KIR gene expression in single-cell RNA-seq data is due to KIR gene misassignment
Eric Alves1,2, Abha Chopra3, Ramesh Ram3
1School of Human Sciences, University of Western Australia, Crawley, Western Australia, Australia.
European Journal of Immunology
|November 9, 2023
Abstract:
Standard single-cell RNA-sequencing alignment pipelines exhibit a propensity for misassigning killer immunoglobulin-like receptor (KIR) transcripts, thereby giving rise to inaccuracies in quantifying KIR expression. Alves et al. elucidated that these default workflows frequently misclassify activating KIR transcripts as inhibitory KIR expression, resulting in a skewed representation of the KIR repertoire.
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