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Fatty acids analysis by capillary electrophoresis: Fundamentals, advantages and applications
Patrícia Abranches Geraldo1, Arthur Woyames de Castro Velasco1, Bruna Marchiori Berlande1
1Grupo de Química Analítica e Quimiometria-GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), Juiz de Fora, Minas Gerais, Brazil.
Abstract:
This review covers the know-how of the Grupo de Química Analítica e Quimiometria regarding the analysis of fatty acids by capillary electrophoresis acquired over its 20 years of existence. Therefore, the fundamentals, advantages, and applications of this technique for analyzing different fatty acids in samples such as food, oils, cosmetics, and biological matrices are presented and discussed. Capillary electrophoresis is, thus, shown as an interesting and valuable separation technique for the target analysis of these analytes as an alternative to the gas chromatography coupled to flame ionization detection, as it offers advantages over the latter such as low analysis times, low sample and reagent consumption, the use of a nondedicated column, and simpler sample preparation. In addition, the methods shown in this literature review can be useful for quality control, adulteration, and health-related research by regulatory agencies.
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