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Enhanced Bonding to Caries-Affected Dentin Using an Isocyanate-Based Primer.
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
A new monomer, ITCM, improves resin bonding to caries-affected dentin (CAD) by creating chemical bonds with collagen. This enhances bond strength and durability, offering a promising solution for dental restorations.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Adhesive Dentistry
Background:
- Dental caries is a prevalent oral disease, frequently necessitating resin restorations.
- Minimally invasive dentistry prioritizes preserving caries-affected dentin (CAD) over complete removal.
- Poor bonding to CAD compromises the longevity and integrity of dental restorations.
Purpose of the Study:
- To evaluate a novel collagen-reactive monomer (ITCM) as a primer for enhancing CAD bonding performance.
- To assess the chemical interactions and bonding efficacy of ITCM with CAD.
- To investigate the impact of ITCM on the durability and quality of the dentin-bonding interface.
Main Methods:
- Fourier transform infrared spectroscopy (ATR-FTIR) to analyze chemical interactions.
- Microtensile bond strength testing after 24-hour water storage and thermocycling.
- Nanoleakage assessment, interfacial nanoindentation, and in situ zymography to evaluate interface quality.
- Cytotoxicity assays to determine ITCM biocompatibility.
Main Results:
- ITCM's isocyanate groups formed covalent and hydrogen bonds with collagen in CAD, facilitating chemical bonding.
- ITCM pretreatment significantly increased CAD bond strength (P < 0.05).
- Improved interfacial sealing, reduced nanoleakage, enhanced hybrid layer homogeneity, and inhibited matrix metalloproteinase activity were observed.
- ITCM demonstrated acceptable biocompatibility.
Conclusions:
- ITCM effectively induces collagen-based chemical bonding in CAD bonding systems.
- ITCM significantly improves immediate and aged bond strength of CAD.
- ITCM shows potential for clinical application in improving dental restorative procedures.
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