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Lithography-free interdigitated electrodes by trench-filling patterning on polymer substrate for Alzheimer's disease
M Mahabubur Rahman1, Nabil H Bhuiyan1, MinJun Park1
1Bio IT Convergence Laboratory, Department of Electronic Convergence Engineering, KwangWoon University, Seoul, 01897, Republic of Korea.
Biosensors & Bioelectronics
|November 13, 2023
Summary
A new, low-cost fabrication method creates interdigitated electrode arrays (IDAs) on polymers. These novel gold IDAs detect Alzheimer's biomarkers amyloid beta 42 (Aß42) and amyloid beta aggregated (Aß Agg.) with high sensitivity.
Area of Science:
- Electrochemistry
- Materials Science
- Biomedical Engineering
Background:
- Conventional microelectrode fabrication methods like lithography are expensive, difficult, and lack reproducibility.
- Interdigitated electrode arrays (IDAs) are crucial electrochemical tools but face fabrication challenges.
- Developing cost-effective and reliable fabrication techniques for IDAs is essential for broader applications.
Purpose of the Study:
- To develop a novel, low-cost patterned-replication fabrication technology for gold interdigitated electrode arrays (IDAs) on polymer substrates.
- To demonstrate the application of the fabricated IDA electrodes for the sensitive detection of Alzheimer's disease biomarkers.
- To establish a reproducible and scalable method for producing gold IDA electrodes.
Main Methods:
- Fabrication of a nickel IDA master mold using UV-lithography on a stainless-steel substrate.
- Replication of the IDA pattern onto a polymer substrate via hot-embossing.
- Deposition of gold onto the replicated polymer using electron beam evaporation.
- Lift-off technique using adhesive tape to obtain the final gold IDA electrode.
Main Results:
- The developed method successfully fabricated gold IDA electrodes on a polymer substrate.
- The IDA electrode achieved a low detection limit of 0.01 nM for p-aminophenol (PAP).
- The fabricated IDA electrode detected Alzheimer's biomarkers amyloid beta 42 (Aß42) and amyloid beta aggregated (Aß Agg.) with low detection limits of 3.9 pg/ml and 7.81 pg/ml, respectively, using e-ELISA.
Conclusions:
- The novel patterned-replication technology offers a low-cost, reproducible, and scalable method for fabricating gold IDA electrodes on polymers.
- The developed gold IDA electrodes are highly sensitive and suitable for detecting Alzheimer's disease biomarkers.
- This technology has the potential to advance electrochemical sensing for disease diagnostics.
