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Effects of resveratrol on DLD and NDUFB9 decrease in frozen semen of Mongolian sheep
Yuting Chen1, Fanhua Meng1, Yang Liu1
1College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Autonomous Region Key Laboratory of Biomanufacturing, Hohhot, 010018, China; Inner Mongolia Endemic Livestock Biotechnology Innovation Team, China.
Cryobiology
|November 13, 2023
Summary
Resveratrol protects Mongolian sheep sperm during cryopreservation by increasing key energy and ROS-related proteins, enhancing sperm vitality. This study identifies DLD and NDUFB9 as crucial proteins affected by freezing.
Area of Science:
- Animal Science
- Reproductive Biology
- Proteomics
Background:
- Cryopreservation of Mongolian sheep sperm is vital for breeding but often reduces sperm vitality and alters morphology.
- Understanding molecular changes during sperm cryopreservation is crucial for improving outcomes.
Purpose of the Study:
- To investigate the impact of resveratrol on cryopreserved Mongolian ram sperm.
- To identify key proteins affected by cryopreservation and the protective role of resveratrol.
Main Methods:
- Sperm samples from Mongolian rams were analyzed in four groups: fresh, frozen without resveratrol, with resveratrol before freezing, and with resveratrol after thawing.
- Proteomic analysis using tandem mass tags (TMT) peptide labeling and LC-MS/MS was performed on fresh and frozen sperm.
- Bioinformatics analysis identified differentially abundant proteins (DAPs) and associated pathways.
Main Results:
- Cryopreservation led to significant changes in protein expression, with 41 upregulated and 48 downregulated proteins identified.
- Key proteins involved in the tricarboxylic acid cycle (TCA) and oxidative phosphorylation, such as dihydrolipoamide dehydrogenase (DLD) and NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9), showed decreased levels.
- Resveratrol supplementation increased the expression levels of DLD and NDUFB9 proteins in cryopreserved sperm.
Conclusions:
- DLD and NDUFB9 are identified as key proteins contributing to reduced vitality in cryopreserved Mongolian ram sperm.
- Resveratrol enhances the levels of DLD and NDUFB9, thereby improving the vitality of cryopreserved Mongolian ram sperm.
- This is the first proteomic study on Mongolian ram sperm cryopreservation, highlighting resveratrol's protective effects.
