Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

47
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
47
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-fidelity fast fluorescence lifetime imaging by event-based denoising.

Nature biotechnology·2026
Same author

High numerical aperture confocal volumetric mesoscope reveals mesoscale subcellular dynamics in vivo.

Nature biotechnology·2026
Same author

Small language models in medicine.

Nature biomedical engineering·2026
Same author

Real-time robust autofocus method enabling sustained intravital scanning light field imaging.

Nature communications·2026
Same author

A multi-modal foundation model for brain disease diagnosis and medical imaging.

Patterns (New York, N.Y.)·2026
Same author

GPI‑anchored protein nanoclusters license migrasome expansion and serve as exocytic platforms for migrasome.

Nature communications·2026

Related Experiment Video

Updated: Jul 11, 2025

Long-term Imaging Mammalian Cells using Wide-Field Microscopy
15:00

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

5.3K

Bio-friendly long-term subcellular dynamic recording by self-supervised image enhancement microscopy.

Guoxun Zhang1,2, Xiaopeng Li3, Yuanlong Zhang1,2

  • 1Department of Automation, Tsinghua University, Beijing, China.

Nature Methods
|November 13, 2023
PubMed
Summary

DeepSeMi, a novel self-supervised learning framework, significantly enhances fluorescence microscopy image quality by reducing noise. This breakthrough allows for clearer, high-resolution imaging of dynamic cellular processes with reduced phototoxicity.

More Related Videos

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

4.8K
Author Spotlight: Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging
04:11

Author Spotlight: Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging

Published on: June 30, 2023

1.6K

Related Experiment Videos

Last Updated: Jul 11, 2025

Long-term Imaging Mammalian Cells using Wide-Field Microscopy
15:00

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

5.3K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

4.8K
Author Spotlight: Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging
04:11

Author Spotlight: Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging

Published on: June 30, 2023

1.6K

Area of Science:

  • Cell biology
  • Microscopy
  • Image processing

Background:

  • Fluorescence microscopy is crucial for observing dynamic cellular processes.
  • Image quality is limited by stochastic noise, affecting resolution and phototoxicity.
  • Existing methods struggle to balance high frame rates, long-term recording, and low phototoxicity.

Purpose of the Study:

  • To develop a self-supervised learning framework for denoising fluorescence microscopy images.
  • To improve signal-to-noise ratio (SNR) without compromising spatiotemporal resolution.
  • To enable high-fidelity imaging of sensitive biological samples under demanding conditions.

Main Methods:

  • Developed DeepSeMi, a self-supervised learning denoising framework.
  • Introduced novel eccentric blind-spot convolution filters.
  • Integrated DeepSeMi with confocal microscopy for advanced imaging.

Main Results:

  • Achieved over 12 dB SNR improvement across diverse imaging conditions.
  • Enabled four-color imaging of organelle interactions at high frame rates.
  • Facilitated long-term monitoring of cellular structures and imaging of phototoxicity-sensitive cells.

Conclusions:

  • DeepSeMi effectively denoises fluorescence microscopy images, overcoming the shot-noise limit.
  • The framework is versatile, biocompatible, and enhances imaging capabilities.
  • DeepSeMi represents a significant advancement for live-cell imaging and dynamic process observation.