Related Experiment Video
Updated: Jul 11, 2025

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
Scattering of Attosecond Laser Pulses on a DNA Molecule during Its Nicking and Bending
Dmitry Makarov1, Anastasia Kharlamova1
1Department of Fundamental and Applied Physics, Northern (Arctic) Federal University, Nab. Severnoi Dviny 17, 163002 Arkhangelsk, Russia.
Abstract:
It is well known that X-ray crystallography is based on X-ray diffraction (XRD) for atoms and molecules. The diffraction pattern arises as a result of scattering of incident radiation, which makes it possible to determine the structure of the scattering substance. With the advent of ultrashort radiation sources, the theory and interpretation of X-ray diffraction analysis have remained the same. This work shows that when an attosecond laser pulse is scattered on a DNA molecule, including during its nicking and bending, the pulse duration is an important characteristic of the scattering. In this case, the diffraction pattern changes significantly compared to the previously known scattering theory. The results obtained must be used in XRD theory to study DNA structures, their mutations and damage, since the previously known theory can produce large errors and, therefore, the DNA structure can be "decoding" incorrectly.
Related Concept Videos
Single-Strand DNA Binding Proteins
Lagging Strand Synthesis
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
Homologous Recombination
Nucleotide Excision Repair
Base Excision Repair
The first step of...

