Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Acrylamide formation in table olives.

Food chemistry·2026

Same author

Effect of packing conditions on the acrylamide content in black ripe olives.

Journal of the science of food and agriculture·2026

Same author

Genome sequences of <i>Lactiplantibacillus pentosus</i> isolated from Spanish and Greek table olive fermentations.

Microbiology resource announcements·2026

Same author

Formation of acrylamide in the presence of quinones.

Food chemistry·2025

Same author

Potassium Hydroxide Extraction of Polyphenols from Olive Leaves: Effect on Color and Acrylamide Formation in Black Ripe Olives.

Foods (Basel, Switzerland)·2024

Same author

LP309 a new strain of Lactiplantibacillus pentosus that improves the lactic fermentation of Spanish-style table olives.

Journal of food science·2023

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

New and Rapid Analytical Method Using HPLC-MS Detection for Acrylamide Determination in Black Ripe Olives.

Mercedes Brenes-Álvarez¹, Eva María Ramírez¹, Manuel Brenes¹

¹Food Biotechnology Department, Instituto de la Grasa (IG, CSIC), Building 46, Ctra. Utrera km 1, 41013 Seville, Spain.

Foods (Basel, Switzerland)

|November 14, 2023

Summary

This summary is machine-generated.

A new, rapid analytical method accurately quantifies acrylamide, a carcinogen, in black ripe olives. This faster technique aids food safety by enabling routine testing for acrylamide levels.

Keywords:

HPLC-MS acrylamide black olive liquid extraction validation

More Related Videos

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis

Published on: December 8, 2023

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Related Experiment Videos

Last Updated: Jul 2, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis

Published on: December 8, 2023

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Area of Science:

Food Chemistry
Analytical Chemistry
Toxicology

Background:

Acrylamide, a known human carcinogen, is present in heated foods, raising consumer safety concerns.
Accurate analytical methods are crucial for monitoring and controlling acrylamide levels in food products.

Purpose of the Study:

To develop and validate a rapid, simple, reliable, and low-cost analytical method for acrylamide quantification in black ripe olives.
To assess the feasibility of this method for routine food safety analysis.

Main Methods:

Water extraction of acrylamide from crushed olives with (13C3)acrylamide as an internal standard.
Quantification using high-pressure liquid chromatography (HPLC) with positive electrospray ionization mass detection.
Method validation including limits of detection (LOD) and quantification (LOQ), accuracy, and precision.

Main Results:

The method achieved an LOD of 4 µg/kg and an LOQ of 11 µg/kg.
Demonstrated high accuracy (98.4-104.8%) and excellent precision (intra- and inter-day <20%).
Analyzed 15 commercial black ripe olive samples, finding acrylamide levels ranging from 79 to 1068 µg/kg.

Conclusions:

The developed HPLC-MS method is a fast (1 hour/sample), accurate, and reliable tool for acrylamide analysis in black ripe olives.
This method offers a significant time advantage over traditional gas chromatography methods.
The protocol's efficiency suggests its potential for routine analysis and extension to other food matrices.