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A Latent Activated Olfactory Stem Cell State Revealed by Single-Cell Transcriptomic and Epigenomic Profiling.
Koen Van den Berge1,2, Dana Bakalar3, Hsin-Jung Chou4
1Department of Statistics, University of California, Berkeley, CA.
Biorxiv : the Preprint Server for Biology
|November 14, 2023
Summary
Scientists discovered a "latent activated" state in olfactory stem cells. These quiescent cells are epigenetically primed, ready to regenerate the olfactory epithelium after injury, aiding neural regeneration research.
Area of Science:
- Neuroscience
- Stem Cell Biology
- Regenerative Medicine
Background:
- The olfactory epithelium exhibits lifelong neurogenesis, making it a model for neural regeneration.
- Studying its stem cells offers insights into neural repair mechanisms after injury.
Purpose of the Study:
- To identify molecular and epigenetic factors governing olfactory stem cell fate during regeneration.
- To understand the heterogeneity of stem cells during injury-induced repair.
Main Methods:
- Single-cell RNA sequencing and ATAC-sequencing were employed.
- Lineage-traced olfactory stem cells were analyzed for gene expression and chromatin accessibility.
Main Results:
- Transcriptional heterogeneity was observed in activated stem cells during fate specification.
- A subset of resting stem cells showed accessible chromatin for wound response genes, indicating pre-activation.
Conclusions:
- Evidence suggests a latent activated stem cell state in the olfactory epithelium.
- Quiescent stem cells can be epigenetically primed for rapid regeneration upon injury.
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