Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenic CD8+ T cells target K71+ Henle's layer by forming cytolytic immune synapses in Alopecia Areata.

Research square·2026
Same author

CXCR4 coordinates adhesion, migration, and development of human NK cells.

bioRxiv : the preprint server for biology·2026
Same author

Autosomal dominant CDC45 deficiency with allelic expression bias causes a novel genetic disease of the immune system.

bioRxiv : the preprint server for biology·2026
Same author

Fibroblast activation protein promotes natural killer cell invasion and tumor infiltration.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Systematic analysis of cellular cross-talk reveals a role for SEMA6D-TREM2 regulating microglial function in Alzheimer's disease.

Science translational medicine·2025
Same author

Joint representation and visualization of derailed cell states with Decipher.

Genome biology·2025
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jul 11, 2025

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

6.0K

cellPLATO: an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data.

Michael J Shannon1, Shira E Eisman1, Alan R Lowe2

  • 1Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York NY 10032.

Biorxiv : the Preprint Server for Biology
|November 14, 2023
PubMed
Summary
This summary is machine-generated.

New software cellPLATO analyzes cell behavior from microscopy data. It reveals Interleukin-15 (IL-15) enhances Natural Killer (NK) cell migration plasticity on ICAM-1 and VCAM-1.

Keywords:
IL-15NK cellscell migrationcell morphologydata-driven analysisintegrinsphenomicsunsupervised machine learning

More Related Videos

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion
09:03

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Published on: April 13, 2019

8.2K
Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

23.8K

Related Experiment Videos

Last Updated: Jul 11, 2025

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

6.0K
Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion
09:03

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Published on: April 13, 2019

8.2K
Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

23.8K

Area of Science:

  • Cell biology
  • Bioinformatics
  • Immunology

Background:

  • Microscopy generates large datasets requiring advanced analysis tools.
  • Phenotypic analysis of cell behavior is crucial for understanding biological processes.

Approach:

  • Introduced cell PLasticity Analysis TOol (cellPLATO), a Python-based software for analyzing cell morphology and motility.
  • Utilized dimensionality reduction and clustering to classify cell behaviors and track changes over time.
  • Applied cellPLATO to investigate Interleukin-15 (IL-15) effects on Natural Killer (NK) cell migration on ICAM-1 and VCAM-1.

Key Points:

  • cellPLATO identifies distinct cell behaviors and behavioral trajectories from live cell microscopy.
  • Analysis revealed 8 behavioral subsets and 4 distinct trajectories for NK cells.
  • IL-15 was shown to increase plasticity in NK cell migration behaviors.

Conclusions:

  • cellPLATO provides a robust method for phenotyping cell behavior from complex microscopy data.
  • IL-15 modulates NK cell migration, increasing behavioral plasticity.
  • Specific integrin ligands (ICAM-1, VCAM-1) differentially influence NK cell migration patterns.