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A strong case for third-party testing

Fridtjof Lund-Johansen1

  • 1Department of Immunology, Oslo University Hospital, Oslo, Norway.

Elife
|November 14, 2023
PubMed

A strategy to identify high-quality commercially available antibodies for research reveals extensive use of non-specific antibodies and offers solutions for future large-scale testing.

Keywords:
antibodyantibody specificityantibody validationbiochemistrycell biologychemical biologyhumanpolyclonal antibodyrecombinant antibody

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Cochran's Q Test is a nonparametric statistical test used to determine if there are potential differences in the outcomes of three or more related groups on a binary (yes/no) or dichotomous outcome. It is essentially an extension of the McNemar Test, which is limited to two related samples - Cochran's Q test can handle three or more related samples, making it more versatile in scenarios where subjects are measured under multiple conditions. The test statistic follows a Chi-Square...
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