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Chicken egg white precipitates induced by water dilution
Bingye Zhao1, Jing Pu1, Gan Hu1
1Institute for Egg Science and Technology, School of Food and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu 610106, China.
International Journal of Biological Macromolecules
|November 15, 2023
Summary
Water dilution of chicken egg whites causes significant precipitation, particularly from thick egg white (TKEW). Lysozyme and mucin-5B are key proteins contributing to this egg white precipitation.
Area of Science:
- Food Science
- Protein Chemistry
- Biochemistry
Background:
- Chicken egg white (EW) undergoes turbidity and precipitation upon dilution with deionized water.
- Precipitation is more pronounced in thick egg white (TKEW) compared to thin egg white (TNEW) and whole egg white.
- Understanding egg white precipitation is crucial for processing and product development.
Purpose of the Study:
- To systematically investigate the formation, characteristics, and composition of egg white precipitates induced by water dilution.
- To identify key proteins responsible for precipitation in different egg white fractions.
- To provide a theoretical basis for reducing water-induced egg white precipitation.
Main Methods:
- Dilution of whole egg white, thick egg white, and thin egg white with deionized water.
- Gravimetric analysis of precipitate formation.
- Quantitative proteomic analysis to identify differentially abundant proteins.
Main Results:
- Thick Egg White Precipitates (TKEWP) constituted 14.47% of TKEW dry matter, significantly higher than other fractions.
- Quantitative proteomics identified 27 differentially abundant proteins (p < 0.05).
- Lysozyme abundance was higher in TNEWP and TKEWP, while mucin-5B showed the highest abundance in TKEWP.
Conclusions:
- Lysozyme is a key protein in egg white precipitation induced by water dilution.
- The insolubility of mucin-5B significantly contributes to the substantial TKEWP formation.
- This study offers novel insights into egg white processing characteristics and precipitation mechanisms.
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