Measurement of unidirectional transplacental flux: a simplified method

A simple method for determining unidirectional transplacental flux in the in vitro perfusion (transfer factor analysis) has been derived from a general treatment of non-compartmental analysis describing transfer between two accessible pools. This method was validated by comparison of the unidirectional transfer fractions for Evans Blue in a two-pool hydraulic model with the true transfer fractions determined from the pump flow rates in the model. There was excellent agreement between calculated and true transfer fractions. Transfer fractions obtained using this method were also compared to the fractions determined by a previously described technique, deconvolution analysis, for a hydraulic model in which a third, inaccessible pool was interposed between the two accessible pools. Good agreement was found between the two methods. Similar agreement was found for the fractional transfer of [14C]L-lactate in the in vitro perfused human placenta, calculated using transfer factor and deconvolution analysis. The sample collection and data processing are much simpler using the former method but the quality of information obtained is reduced accordingly.